WARNINGNever perform a venipuncture with thepatient standing up. If patients should faint,they could seriously injure themselves.Also, safeguards should be in place toprevent patients from falling forward whenthey are seated.To perform venipuncture, follow the steps givenbelow.1. Explain the procedure to the patient.2. Apply tourniquet around the arm approximately2 to 3 inches above the antecubital fossa (thedepression in the anterior region of the elbow,see figure 7-4) with enough tension so that theVEIN is compressed, but not the ARTERY. ABP cuff (sphygmomanometer) may be usedinstead of a tourniquet if a patient is difficult todraw.3. Position the patient’s arm extended with little orno flexion at the elbow.4. Locate a prominent vein by palpation (feeling).If the vein is difficult to find, it may be mademore prominent by massaging the arm with anupward motion to force blood into the vein.5. Cleanse the puncture site with a 70% alcoholpad or povidone-iodine solution and allow todry.CAUTION: After cleaning the puncturesite, only the sterile needle should be allowedto touch it.6. “Fix” or hold the vein taut. This is bestaccomplished by placing the thumb under thepuncture site and exerting a slight downwardpressure on the skin or placing the thumb to theside of the site and pulling the skin taut laterally(fig. 7-4).7. Using a smooth continuous motion, introducethe needle, bevel side up, into the side of the veinat about a 15-degree angle with the skin(fig. 7-4).8. Holding the vacutainer barrel with one hand,push the tube into the holder with the other handand watch for the flow of blood into the tubeuntil filling is completed.9. Once all the specimens have been collected,hold the vacutainer with one hand and releasethe tourniquet with the other.10. Place a sterile gauze over the puncture site andremove the needle with a quick, smooth motion.11. Apply pressure to the puncture site and instructthe patient to keep the arm in a straight position.Have the patient hold pressure for at least 3minutes.12. Take this time to invert any tubes that need tohave anticoagulant mixed with the blood.13. Label specimens.14. Reinspect the puncture site to make surebleeding has stopped, and apply a bandage.7-6NEEDLE BEVEL UPSKIN15OANTECUBITALFOSSAHM3f0704VEINFigure 7-4.—Venipuncture.
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