at 100 rpm. To help differentiate nonreactive
from reactive results, you should briefly rotate
and tilt the card by hand (3 or 4 back-and-forth
motions).
10. Immediately read the card macroscopically
(with the unaided eye) in the wet state under a
high-intensity lamp.
11. Compare the patients tests to the controls for
correct interpretations. The reactive control
should show small to large clumps. The
nonreactive control should show no clumping or
very slight roughness. The reactive-minimal-
to-moderate control should show slight but
definite clumping.
12. Report the test as
reactive, if agglutination or flocculation is
present, or
nonreactive, if no agglutination is present.
NOTE: The RPR Card test is used as a
screen for syphilis. If a patients RPR is
reactive, the patient should be sent to a
laboratory to have a FTA-ABS
(Fluorescent Treponemal Antibody
Absorption Test) performed. The
FTA-ABS, a more precise test, is used to
confirm primary, secondary, and late
syphilis.
MONOSTICON DRI-DOT SLIDE
TEST
Mononucleosis imitates many diseases so well that
diagnosis is confirmed only by selective serologic
testing. The Monosticon DRI-DOT Slide Test is an
accurate, 2-minute disposable test designed to detect
the presence of infectious mononucleosis antibodies in
serum, plasma, or whole blood.
Principle of the Monosticon DRI-DOT
Slide Test
The Monosticon DRI-DOT Slide Test consists of
specially prepared, stable sheep and/or horse
erythrocyte antigen (dyed) and guinea pig antigen on a
disposable slide. When serum, plasma, or whole blood
is mixed with these antigens on the slide, the test result
for infectious mononucleosis will be positive or
negative.
A positive result is indicated by
agglutination and a negative result is indicated by no
agglutination.
Materials Required for Monosticon
DRI-DOT Slide Test
To perform the Monosticon DRI-DOT Slide Test,
the following materials are required:
Serum or plasma specimen
Monosticon DRI-DOT Test kit, which consists
of:
Monosticon DRI-DOT Test slides
Positive I.M. (infectious mononucleosis)
serum control
Negative I.M. serum control
Dropper bottle
Dispenstirs® (designed to deliver a 0.03 ml
drop)
Distilled water
Centrifuge
DRI-DOT slide holder (available commercially,
but not necessary to perform test)
Controls for Monosticon DRI-DOT
Slide Test
Both a positive and negative control are included
in each kit to check the effectiveness of the reagents.
The positive I.M. serum control (human) is a dilution
of human sera (sing. serum) containing the specific
heterophile antibody of infectious mononucleosis.
The negative I.M. serum control (human) is a dilution
of human sera containing no detectable antibody to
infectious mononucleosis. Both controls have been
dried and placed in a vial with color-coded cap and
label. Since both controls are of human origin, they are
potentially infectious and must be handled with care.
Both controls (positive and negative) should be
tested before performing test with serum, plasma, or
whole blood. Controls are prepared in the same manner
as serum and plasma test described in the next section,
but instead of adding serum or plasma to the slide, the
control is added. Before each control is used, it must be
reconstituted with 0.5 ml of distilled water. If results of
the control tests are not as expected, do not use the test
kit.
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