Figure 10-13. - Burs in glass test tubes.
perform biological monitoring weekly. Figure 10-14 shows a biological incubator with endospore tests.
TEST PROCEDURE. - Since biological monitoring systems are designed for specific sterilization methods, you must be sure to use a system compatible with the sterilization method used. The following test procedures should be used to ensure effectiveness of the sterilization process:
The use of a "test pack" is most practical while processing an instrument pack.
Biological spore strips or ampules should be placed between several layers of folded wrapping material, and then the test pack is double-wrapped in the normal manner.
Always follow the biological monitor manufacturer’s directions for the placement of the test pack within the sterilizer.
As a general rule, the biological spore strips or ampules should be placed within an area of the sterilizer that is least accessible to the sterilizing agent that is being used.
If using steam under pressure sterilizers, place the test pack in the lower front of the sterilization chamber.
If using tabletop units, place the test pack in the center of the load.
For each test, use an unprocessed monitor for a control.
Figure 10-14. - Biological incubator.
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