3. Using the broad end of the dispenstir, mix
until the dehydrated control specimen is dissolved.
Spread specimen over entire area of circle. Use a
separate dispenstir for each circle.
4. To draw the patients sample, hold the
dispenstir between the thumb and forefinger near
stirring or sealed end and squeeze; do not release
pressure until the open end is below the surface
of the specimen. Release finger pressure to draw
up the sample.
5. Hold the dispenstir in a vertical position,
directly over the card test area to which the
specimen is to be delivered; squeeze dispenstir,
allowing 1 drop to fall onto the test area.
6. Invert the dispenstir, and, with the sealed
end, spread specimen within the confines of the
circle. Discard the dispenstir.
7. Continue the above steps until one or two
test cards are filled with patients samples.
8. Gently shake the antigen dispensing bottle
before use. Hold in the vertical position and
dispense several drops into the dispensing bottle
cap to ensure that the needle passage is clear.
Allow 1 free-falling drop to fall onto each
test area. Do not stir; mixing of antigen
suspension and specimen is accomplished during
9. Put card(s) on rotator and cover with
10. Rotate for 8 minutes at 100 r.p.m. Follow-
ing rotation, to help differentiate Nonreactive
from Reactive results, a brief rotating and tilting
of card by hand (3 to 4 to-and-fro motions) must
11. Then immediately read Card micro-
scopically in the wet state and under the
12. The Reactive control should show
characteristic strong clumping; the Nonreactive
control should show smooth, grayish appearance
of unclumped particles. The Reactive Minimal-to-
Moderate control should show minimal-to-
moderate clumping. The patients tests should be
compared to the controls for correct interpreta-
13. Report test as:
a. Reactive if specimen shows aggluti-
nation or flocculation.
b. Nonreactive if specimen shows no
agglutination at the end of 8 minutes rota-
c. If the RPR test is reactive, an
FTA-ABS (Flourescent Treponemal Antibody
Absorption Test) must be run on the speci-
MONOSTICON SLIDE TEST FOR
The main reason for including this test is that
mononucleosis imitates many diseases so well that
diagnosis is confirmed only by selective serologic
Principle of the Test
1. Absorption of serum with a suspension of
a guinea pig or horse kidney antigen removes
antisheep agglutinins in the serum of patients
with serum diseases and various infectious
2. In some serum of patients with infectious
mononucleosis, a substantial part of the
antibodies remains after absorption.
3. Absorption with a suspension of beef cells
removes the antisheep agglutinins in infectious
mononucleosis, but leaves them in other infectious
Rapid slide tests for infectious mononucleosis
are based on these principles. Suspensions of
guinea pig kidney and beef erythrocyte stomata
result in satisfactory instant absorption of
antibodies and clear differentiation between
infectious mononucleosis and noninfectious
mononucleosis sera. Infectious mononucleosis
antibodies may be demonstrated as early as the
fourth day of illness and practically always by the
twenty-first day. Positive results may continue for
1. On a clean slide (supplied with kit) place
1 drop of guinea pig antigen, reagent I, into box
2. Place 1 drop of the beef erythrocyte
stomata, reagent II, into box number 2.
3. Add 1 drop of test serum on plasma to both
boxes. Mix each with separate sticks.
4. Add 1 drop of horse erythrocyte antigen
(supplied with kit) to both boxes. Mix each with
separate disposable sticks.
5. Rock slide back and forth for 2 minutes
so that liquid flows slowly over the entire area of
6. Read results after 2 minutes.
Agglutination in box 1 is positive for
Agglutination in box 2 is positive for
No agglutination in either box is
negative for mononucleosis.