Figure 6-4.Blood cell pipettes.
(mm3) of blood. The normal
is as follows:
adult male 5.4 + .8
red blood cell
adult female 4.8 + .6 million/mm3
newborn 5.1 + .9 million/mm3
A lower count is usually a sign of anemia.
Manual Sahli Pipette Method
The materials listed below are required to per-
form a red blood cell count using the manual Sahli
. Hemacytometer set
Microscope with lamp
Red cell pipette
Hand held counter
Red cell diluting fluid. 0.85 percent sodium
chloride (normal saline) is preferred
because of its ready availability and its
ability to prevent clumping of red cells.
1. Using well mixed anticoagulated blood or
blood directly from a fingerstick, fill the
clean, dry, red cell pipette (fig. 6-4) exactly
to the 0.5 mark with the aid of the suction
tube. Hold the pipette in a nearly horizon-
tal position so that the exact level of the
blood can be seen. The curve of the tip may
rest on the skin, but the orifice must be free
Figure 6-5.Mixing blood and diluting fluid.